FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_23.3510000005d809.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_23.3510000005d809.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30529
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGCTATCCCTGTCTCTTATACACA2190.7173507157129287No Hit
ATCTGCTATCCTGTCTCTTATACACAT650.21291231288283272No Hit
ATCTGCTATCCGGCTGTCTCTTATACA430.14084968390710473No Hit
ATCTGCTATCCGCCTGTCTCTTATACA340.11136951750794327No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCTA250.002918666221.4537812
TATGCTA250.002918666221.4537812
ATCTACT454.857193E-721.4537811
TCTACTA454.857193E-721.4537812
ACCTGCT250.002918666221.4537811
TCTGCTA28050.021.4537812
ATGCTAT250.002918666221.4537813
ATATGCT250.002918666221.4537811
ATTTGCT303.29832E-421.4537811
CTGCTAT28500.021.4537813
ATCTGCT28050.021.4537811
TTTGCTA303.29832E-421.4537812
CTACTAT454.857193E-721.4537813
TTGCTAT303.29832E-421.4537813
ATCCGGC2250.020.9262318
ATCCGCT1250.020.9262318
TGGTTTT1050.020.9262318
GTGTTTT1100.020.9262318
GGGTTTT1700.020.9262318
GCCTTTT507.239214E-820.9262318
Produced by FastQC (version 0.11.2)