FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_21.3510000005d7b7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_21.3510000005d7b7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25593
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTGAACTGGGGCTGTCTCTTATAC670.26179033329426016No Hit
ACCTTGAACTGGGCCTGTCTCTTATAC330.12894150744344157No Hit
ACCTTGAACTGGGTCTGTCTCTTATAC300.11721955222131053No Hit
ACCTTGAACTGGAGCTGTCTCTTATAC270.10549759699917946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTGA505.6197678E-821.3961
CCTTGAA23200.021.3962
TCTTGAA505.6197678E-821.3962
ACCTTGA23200.021.3961
CTTGAAC23700.021.3963
TTTGAAC751.8189894E-1221.3959983
CATTGAA303.3381558E-421.3959982
ACATTGA303.3381558E-421.3959981
ACTTTGA751.8189894E-1221.3959981
ATTGAAC303.3381558E-421.3959983
CTTTGAA751.8189894E-1221.3959982
TTGTTTT405.2148243E-620.93542118
TGGTTTT751.8189894E-1220.93542118
TCCTTTT250.00335066920.93542118
GCGATTT250.00335066920.93542117
AGGATTT250.00335066920.93542117
CTGGGTG800.020.9354219
CTGGGTC800.020.9354219
CTGGGTA507.089875E-820.9354219
AACTGGT5100.020.9354217
Produced by FastQC (version 0.11.2)