FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_19.3510000005d774.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_19.3510000005d774.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39018
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGAGCGCATTCTGTCTCTTATACAC410.10507970680198883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGAGC33700.021.2574791
CTGAGCG800.021.2574772
CAGAGCG33500.021.2574772
TCAGCGC1700.021.2574771
AGAGCGC33950.021.2574773
CGAGCGC1600.021.2574773
CCGAGCG1600.021.2574772
TAGAGCG455.39123E-721.2574772
AGCGCGC1700.021.2574773
CAGCGCG1700.021.2574772
TGAGCGC800.021.2574773
ACTGTTT303.8931423E-420.95769317
GTGTTTT1150.020.95769318
CATTGTT601.0040822E-920.9576939
GGGTTTT1800.020.95769318
CATTGTG1050.020.9576939
AGCTTTT456.1851097E-720.95769318
CATTGGC1350.020.9576939
CATTGGA1100.020.9576939
CATTGCG1100.020.9576939
Produced by FastQC (version 0.11.2)