FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_18.3510000005d75b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_18.3510000005d75b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17106
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGATACCTGTCTCTTATACACA1360.7950426750847657No Hit
GCATTGGATACCGGCTGTCTCTTATAC870.5085934759733427No Hit
GCATTGGATACCCTGTCTCTTATACAC760.4442885537238396No Hit
GCATTGGATACCGTCTGTCTCTTATAC550.32152461124751547No Hit
GCATTGGATACCGCCTGTCTCTTATAC440.2572196889980124No Hit
GCATTGGATACCCGCTGTCTCTTATAC380.2221442768619198No Hit
GCATTGGATACCAGCTGTCTCTTATAC380.2221442768619198No Hit
GCATTGGATACCATCTGTCTCTTATAC350.2046065707938735No Hit
GCATTGGATACCTGCTGTCTCTTATAC350.2046065707938735No Hit
GCATTGGATACCACCTGTCTCTTATAC330.1929147667485093No Hit
GCATTGGATACCCCCTGTCTCTTATAC250.1461475505670525No Hit
GCATTGGATACCCTGTCTCTTCTACAC250.1461475505670525No Hit
GCATTGGATACCGCTGTCTCTTATACA220.1286098444990062No Hit
GCATTGGATACCGACTGTCTCTTATAC220.1286098444990062No Hit
GCATTGGATACCCCTGTCTCTTATACA200.11691804045364199No Hit
GCATTGGATACCTCCTGTCTCTTATAC190.1110721384309599No Hit
GCATTGGATACTGTCTCTTATACACAT180.1052262364082778No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGGA14950.021.376122
GCCTTGG1200.021.376121
ATTGGAT15200.021.376123
GTATTGG250.002926981821.376121
CCTTGGA1200.021.376122
TATTGGA250.002926981821.376122
CTTGGAT1200.021.376123
GCATTGG14950.021.376121
TTGTTTT303.7938973E-420.93859718
TGGTTTT405.0420695E-620.93859718
TCCTTTT250.00330291920.93859718
ACCGGGT250.00330291920.93859710
ACCGGGG405.0420695E-620.93859710
ACCGGGA303.7938973E-420.93859710
ACCGGCT303.7938973E-420.93859710
GTGTTTT250.00330291920.93859718
ACCGGAG303.7938973E-420.93859710
GGGTTTT651.03682396E-1020.93859718
CAGTTTT354.3694108E-520.93859718
CCGGAGG250.00330291920.93859711
Produced by FastQC (version 0.11.2)