FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_16.3510000005d717.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_16.3510000005d717.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50072
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACACCATAGCCTGTCTCTTATACAC4910.9805879533471802No Hit
GAACACCATAGCGGCTGTCTCTTATAC2540.5072695318741013No Hit
GAACACCATAGCCCCTGTCTCTTATAC1190.2376577728071577No Hit
GAACACCATAGCAGCTGTCTCTTATAC1170.23366352452468447No Hit
GAACACCATAGCGCCTGTCTCTTATAC1160.23166640038344782No Hit
GAACACCATAGCGTCTGTCTCTTATAC1110.22168077967726474No Hit
GAACACCATAGCTGTCTCTTATACACA1080.21568940725355487No Hit
GAACACCATAGCACCTGTCTCTTATAC990.19771528998242532No Hit
GAACACCATAGCGACTGTCTCTTATAC830.1657613037226394No Hit
GAACACCATAGCATCTGTCTCTTATAC810.16176705544016617No Hit
GAACACCATAGCTGCTGTCTCTTATAC760.15178143473398306No Hit
GAACACCATAGCCGCTGTCTCTTATAC710.14179581402779998No Hit
GAACACCATAGCCCTGTCTCTTATACA680.1358044416040901No Hit
GAACACCATAGCTCCTGTCTCTTATAC680.1358044416040901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCACCA2450.021.3109762
ACACCAT43700.021.3109763
GTACACC701.0913936E-1121.3109761
AACCCCA1800.021.3109762
TACACCA701.0913936E-1121.3109762
CCACCAT2450.021.3109763
GAACCCC1800.021.3109761
ACCCCAT1800.021.3109763
GAACACC42900.021.3109761
GATCACC751.8189894E-1221.3109761
ATCACCA751.8189894E-1221.3109762
TCACCAT751.8189894E-1221.3109763
GACCACC2450.021.3109761
AACACCA42900.021.3109762
TAGCGGG3400.020.9490539
TCCTTTT354.5756064E-520.94905318
GGTGATT354.5756064E-520.94905316
TAGCGAC701.4551915E-1120.9490539
ATCTTTT354.5756064E-520.94905318
AGCTTTG558.716597E-920.94905310
Produced by FastQC (version 0.11.2)