FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_15.3510000005d6fc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_15.3510000005d6fc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28886
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCATAGCTGCTGTCTCTTATACACA3021.0454891643010455No Hit
TGGCATAGCTGAGGGGCTGTCTCTTAT740.25617946410025616No Hit
TGGCATAGCTGAGGCTGTCTCTTATAC650.22502250225022502No Hit
TGGCATAGCTGAGGGGGGGGTTTTTTT420.1453991553001454No Hit
TGGCATAGCTGAGAGGCTGTCTCTTAT340.1177040781001177No Hit
TGGCATAGCTGAGGGCCTGTCTCTTAT330.11424219345011424No Hit
TGGCATAGCTGAGGGCGGGGTTTTTTT320.11078030880011078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCATA25350.021.3823011
GTCATAG556.5683707E-921.3823012
GGCATAG25350.021.3823012
GGCGTAG250.00297204621.3823012
GCCTAGC1400.021.3823013
TGTCATA556.5683707E-921.3823011
GGCCTAG1400.021.3823012
TGGAATA303.3677043E-421.3823011
TGGCGTA250.00297204621.3823011
GAATAGC303.3677043E-421.3823013
GCGTAGC250.00297204621.3823013
GGAATAG303.3677043E-421.3823012
TCATAGC556.5683707E-921.3823013
TGGCCTA1400.021.3823011
GCATAGC25500.021.38233
TGAGACG303.8842534E-420.9376110
TGATGGC303.8842534E-420.9376110
TGGTTTT609.822543E-1020.9376118
GAGGCGA303.8842534E-420.9376111
CTGAGTT303.8842534E-420.937619
Produced by FastQC (version 0.11.2)