FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_14.3510000005d6ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_14.3510000005d6ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27370
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTCTGTTGCTGTCTCTTATACACA3321.2130069419071976No Hit
ATGCTCTGTTGCGGCTGTCTCTTATAC620.226525392765802No Hit
ATGCTCTGTTGCCTGTCTCTTATACAC520.18998903909389844No Hit
ATGCTCTGTTGCGGGGCTGTCTCTTAT470.17172086225794664No Hit
ATGCTCTGTTGCGGGGGGGGTTTTTTT330.1205699671172817No Hit
ATGCTCTGTTGCTGTCTCTTCTACCCA310.113262696382901No Hit
ATGCTCTGTTGCGCCTGTCTCTTATAC300.10960906101571063No Hit
ATGCTCTGTTGCGGGGGGGATTTTTTT290.10595542564852027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACTG404.5661145E-621.2671622
TTCTCTG455.244892E-721.2671622
GGCTCTG404.5661145E-621.2671622
AGGCTCT404.5661145E-621.2671621
ATGCACT404.5661145E-621.2671621
ATTCTCT455.244892E-721.2671621
AAGCTCT250.003062981421.2671621
ATGATCT353.984363E-521.267161
ATGCTCT24650.021.267161
TGCTCTG24750.021.1812322
GCTCTGT25550.021.1423053
ACTGTTG405.192627E-620.9561255
TGTTGCC5750.020.9561257
TTGCATT250.003337490420.9561259
TTGCATC250.003337490420.9561259
GTGTTTT1050.020.95612518
GGGTTTT1450.020.95612518
GCCTTTT405.192627E-620.95612518
TTGCAGC405.192627E-620.9561259
TTGCACG507.062408E-820.9561259
Produced by FastQC (version 0.11.2)