FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_10.3510000005d65c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_10.3510000005d65c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17368
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTCTCTGCATCTGTCTCTTATACAC2181.2551819438046983No Hit
ACGTCTCTGCCTGTCTCTTATACACAT1160.6678949792722249No Hit
ACGTCTCTGCATCTGTCTCTTCTACAC1060.6103178258866881No Hit
ACGTCTCTGCCTGTCTCTTATCCACAT420.2418240442192538No Hit
ACGTATCTGCATCTGTCTCTTCTACAC200.11515430677107323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTC15000.021.2099131
GTATCTG1300.021.2099133
ACGTATC1300.021.2099131
ACTTCTC250.003065595421.2099131
TTCTCTG250.003065595421.2099133
GTCTCTG15300.021.2099133
CGTATCT1300.021.2099132
ATGTCTC303.4783946E-421.2099131
CTTCTCT250.003065595421.2099132
CGTCTCT15000.021.2099132
CTGCATA3500.020.965427
TTGTTTT250.00328041620.96541818
TGGTTTT608.894858E-1020.96541818
CTCTGCA15650.020.9654185
TGCATTT651.0186341E-1020.9654188
TGCATTG1400.020.9654188
TGCATTC751.8189894E-1220.9654188
TGCATTA751.8189894E-1220.9654188
GTGTTTT751.8189894E-1220.96541818
TGCATGT1550.020.9654188
Produced by FastQC (version 0.11.2)