FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_1.3510000005d533.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_1.3510000005d533.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37635
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCTGCTCCTGTCTCTTATACACAT1300.3454231433506045No Hit
GTGTCTGCTCCTGTCTCTTATCCACAT440.11691244851866614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTG404.6856767E-621.2452831
GTGTATG2750.021.2452831
GAGTCTG303.5462304E-421.2452831
GTGTGTG455.410402E-721.2452811
TTTCTGC250.00312756521.216692
GGTCTGC303.5787706E-421.216692
TTCTGCT250.00312756521.216693
GTCTGCT33050.021.216693
AGTCTGC303.5787706E-421.216692
CGTCTGC303.5787706E-421.216692
TGTCTGC32200.021.2166882
TGTTTGC404.740972E-621.2166882
TGTATGC2750.021.2166882
GTGTGCT455.481379E-721.2166883
TGTGTGC455.481379E-721.2166882
GTGTCTG32350.021.1139351
ATGGGTA250.003354499120.96276711
TGGTTTT609.968062E-1020.96276718
ATGGGGT250.003354499120.96276711
ATGGGCT354.5138382E-520.96276711
Produced by FastQC (version 0.11.2)