FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_94.3510000005d4d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_94.3510000005d4d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80469
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTGCAAGGACTGTCTCTTATACACA1860.23114491294784328No Hit
TACTGCAAGGAGGGGGGGCTGTCTCTT980.1217860294026271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTGCA73500.021.4719411
TTCTGCA701.0913936E-1121.471941
TAATGCA850.021.471941
TATTGCA1550.021.471941
TACTCCA556.706614E-921.471941
TACTACA850.021.471941
ACTGCAA73550.021.4582562
CTACAAG850.021.4582543
TTGCAAG1550.021.4582543
TCTGCAA701.0913936E-1121.4582542
ATTGCAA1550.021.4582542
ATGCAAG850.021.4582543
AATGCAA850.021.4582542
CTGCAAG74200.021.4582543
ACTACAA850.021.4582542
CTCCAAG556.7557266E-921.4582523
ACTCCAA556.7557266E-921.4582522
GAGGCGT751.8189894E-1220.92479910
GAGGCCA303.9853193E-420.92479910
GAGGTCA456.453538E-720.92479910
Produced by FastQC (version 0.11.2)