FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_92.3510000005d4ae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_92.3510000005d4ae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44741
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACA1900.021.2656081
AGCCACT39800.021.2656083
TTCGCCA1550.021.2656081
CGCCACT1550.021.2656083
GAGCCAC608.458301E-1021.2656082
TCGCCAC1550.021.2656082
TGAGCCA608.458301E-1021.2656081
TAGACAC1900.021.2656082
TTTGCCA608.458301E-1021.2656081
TTGCCAC608.458301E-1021.2656082
TAGCCAC38950.021.2656062
TTAGCCA38950.021.2656061
CTCTGGT4200.020.9563778
CTCTGGC4200.020.9563778
CTCTGGA2950.020.9563778
CTCTGCT1850.020.9563778
CTCTGCC1850.020.9563778
GAGGTCT303.9071622E-420.95637717
GCCTTTT558.618372E-920.95637718
GTCATTT303.9071622E-420.95637717
Produced by FastQC (version 0.11.2)