FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_90.3510000005d46a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_90.3510000005d46a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29674
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGAGA250.00306052621.2773973
TCCCAGA404.568865E-621.2773971
TCAAGAG250.00306052621.2773972
CCCAGAG404.568865E-621.2773972
TTCAAGA250.00306052621.2773971
CCAGAGA26100.021.2773973
TTTCAGA455.2519135E-721.2773971
TTCCCGA1900.021.2773971
TCAGAGA455.2519135E-721.2773973
CCCGAGA1900.021.2773973
TCCCGAG1900.021.2773972
TTCAGAG455.2519135E-721.2773972
TACCAGA250.00306052621.2773971
ACCAGAG250.00306052621.2773972
GCCAGAG303.4854206E-421.2773952
TCCAGAG25200.021.235182
TTCCAGA25150.021.2350981
TTGTTTT651.1459633E-1020.9544718
ACTGCGG651.1459633E-1020.954479
ACTGCGC456.0909406E-720.954479
Produced by FastQC (version 0.11.2)