FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_88.3510000005d427.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_88.3510000005d427.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22833
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGCTTGACCTGTCTCTTATACAC1300.5693513773923707No Hit
GTTGAGCTTGACCTGTCTCTTCTACAC400.17518503919765252No Hit
GTTGAGCTTGACGCTGTCTCTTATACA350.15328690929794594No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAGC250.003035989121.2806241
GTTGAGC18300.021.2806241
TTGAGCT18300.021.2806242
TAGAGCT250.003035989121.2806242
TTGCGCT2050.021.2806242
GTTGCGC2050.021.2806241
CTGAGCT303.4470865E-421.2806222
GTCGAGC404.4850112E-621.2806221
TGGAGCT607.748895E-1021.2806222
GCTGAGC303.4470865E-421.2806221
GTGGAGC607.748895E-1021.2806221
TGAGCTT18800.021.2806223
GGAGCTT607.748895E-1021.2806223
TCGAGCT404.4850112E-621.2806222
GATTTTT1950.020.95394919
CTTGACA4600.020.9539497
TGACGGG1950.020.9539499
TTGTTTT405.134376E-620.95394718
TGGTTTT800.020.95394718
GTGTTTT609.422365E-1020.95394718
Produced by FastQC (version 0.11.2)