FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_87.3510000005d40d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_87.3510000005d40d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37834
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCGTCAGCCTGTCTCTTATACACA520.13744251202621982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTCA32450.021.2412873
ACGTCGT900.021.2412871
GTCGTCA900.021.2412873
CCTCGTC557.2777766E-921.2412872
ACATCGT404.6944497E-621.2412871
ACTTCGT32000.021.2412871
ACTTAGT2500.021.2412871
ACCTCGT557.2777766E-921.2412871
CGTCGTC900.021.2412872
ATCGTCA404.6944497E-621.2412873
ATTTCGT404.6944497E-621.2412871
TTAGTCA2500.021.2412873
CTCGTCA557.2777766E-921.2412873
TTTCGTC404.6944497E-621.2412872
CATCGTC404.6944497E-621.2412872
CTTCGTC32100.021.20822
CGTCAGC34850.020.960325
CAGCTTC1650.020.960328
CAGCTCT1900.020.9603188
CAGCTCG2500.020.9603188
Produced by FastQC (version 0.11.2)