FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_85.3510000005d3c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_85.3510000005d3c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31781
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGTACCGCTGTCTCTTATACACA1160.36499795475283975No Hit
CTCTAGTACCGAGGCTGTCTCTTATAC340.1069821591516944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGT404.5603156E-621.29121
CTCTAGT27750.021.29121
CACTAGT303.477775E-421.29121
CTTTAGT506.0415914E-821.2911991
TCTAGTA27800.021.2571892
GCTAGTA250.003082364821.2571892
TCTCGTA1600.021.2571892
ATAGTAC404.6243404E-621.2571893
TTTAGTA506.145456E-821.2571892
CTAGTAC28450.021.2571893
TATAGTA404.6243404E-621.2571892
TTAGTAC506.145456E-821.2571893
CTCGTAC1600.021.2571893
ACTAGTA303.5157218E-421.2571872
TTGTTTT558.383722E-920.95590618
TCCTTTT303.873307E-420.95590618
GGGTCTG303.873307E-420.95590618
GGGTTTT1300.020.95590618
GAGCGGG250.00334946220.95590611
CAGTTTT250.00334946220.95590618
Produced by FastQC (version 0.11.2)