FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_84.3510000005d3af.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_84.3510000005d3af.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43494
Sequences flagged as poor quality0
Sequence length27
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAACGACAGCTGTCTCTTATACACA1750.40235434772612316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG1800.021.2118761
CAGAACG38200.021.2118761
CTGAACG557.4742275E-921.2118741
CATAACG303.5969354E-421.2118741
CAGCACG1650.021.2118721
TAACGAC303.6254124E-421.187213
ATAACGA303.6254124E-421.187212
AGAACGA38450.021.187212
GACCGAC1800.021.187213
AGACCGA1800.021.187212
TGAACGA557.576091E-921.187212
GAACGAC39000.021.1872083
GCACGAC1650.021.1872083
AGCACGA1650.021.1872082
ACAGTAA900.020.9677818
AGTAGGG456.190221E-720.96778110
TGATTTT456.190221E-720.96778118
AGTGGGT900.020.96778110
AGTGGCA456.190221E-720.96778110
GGTTTTT3500.020.96778119
Produced by FastQC (version 0.11.2)