FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_82.3510000005d36b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_82.3510000005d36b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59877
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCGTCTCGATCTGTCTCTTATACAC2200.3674198774153682No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCTC354.0325802E-521.3058623
ACACGTC455.374295E-721.3058621
ACTCGTC54200.021.3058621
ACGTCTC455.374295E-721.3058623
TTCGTCT455.374295E-721.3058622
CTCCTCT354.0325802E-521.3058622
ACTCCTC354.0325802E-521.3058621
ACCCGTC1100.021.3058621
CCGTCTC1100.021.3058623
TAGTCTC354.0325802E-521.3058623
ACTCATC701.0913936E-1121.3058621
CCCGTCT1100.021.3058622
TCGTCTC54650.021.3058623
GCGTCTC1150.021.3058623
CTAGTCT354.0325802E-521.3058622
ACTAGTC354.0325802E-521.3058621
TCATCTC701.0913936E-1121.3058623
CTCATCT701.0913936E-1121.3058622
ACGCGTC1150.021.3058621
CGCGTCT1150.021.3058622
Produced by FastQC (version 0.11.2)