FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_81.3510000005d342.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_81.3510000005d342.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19805
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGGAATGGCCTGTCTCTTATACACA420.2120676596818985No Hit
AACGGAATGGCTGCTGTCTCTTATACA360.18177227972734158No Hit
AACGGAATGGCTGTCTCTTATACACAT280.14137843978793235No Hit
AACGGAATGGCTGGGGGCTGTCTCTTA250.12623074981065388No Hit
AACGGAATGGCTCTGTCTCTTATACAC220.1110830598333754No Hit
AACGGAATGGCTCCTGTCTCTTATACA200.10098459984852311No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAA250.00294769821.3711341
AACGAAA250.00294769821.3711341
ACGCAAT250.00294769821.3711342
CGGAATG18650.021.3711343
CGAAATG250.00294769821.3711343
ACGAAAT250.00294769821.3711342
CGCAATG250.00294769821.3711343
AACGGAA18600.021.3711321
ACGGAAT18600.021.3711322
GTTCTGT303.8225774E-420.93939620
GCTGGAG303.8225774E-420.93939610
GGCTGCG609.276846E-1020.9393969
GGCTGCC303.8225774E-420.9393969
GAGTTTT303.8225774E-420.93939618
GCTGTGC303.8225774E-420.93939610
GCTCGGG303.8225774E-420.93939610
GTCTTTT303.8225774E-420.93939618
GGCTCGA303.8225774E-420.9393969
GGCTCCC303.8225774E-420.9393969
GGCTTAG303.8225774E-420.9393969
Produced by FastQC (version 0.11.2)