FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_79.3510000005d2f0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_79.3510000005d2f0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36385
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACATCGCAAAAAAAAAAAAAAAAA720.1978837433008108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACAT404.865842E-621.1497941
TACATCG608.894858E-1021.1497943
GACATCG32700.021.1497943
ATACATC608.894858E-1021.1497942
CATACAT608.894858E-1021.1497941
CAGCCAT850.021.1497921
AGCCATC850.021.1497922
GAAATCG1400.021.1497923
AGAAATC1400.021.1497922
GCCATCG850.021.1497923
CAGACAT32400.021.1497921
CAGAAAT1400.021.1497921
AGACATC32450.021.1172052
TGGTTTT950.020.97524318
GCAATGA950.020.9752439
ACATCGC33700.020.9752434
TCGCAAC7050.020.9752437
CAAGGGG950.020.97524310
CGCAAAC1900.020.9752438
CGTTTTT1900.020.97524319
Produced by FastQC (version 0.11.2)