FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_78.3510000005d2d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_78.3510000005d2d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25924
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGTGACGAACCTGTCTCTTATACAC1420.5477549760839376No Hit
AGTGTGACGAACCTGTCTCTTCTACAC430.16586946458879803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGACG23450.021.53
GGTGACG353.6510995E-521.53
GAGTGAC505.3429176E-821.52
TGCGACG250.002870283321.53
AGGGTGA353.6510995E-521.51
AGAGTGA505.3429176E-821.51
AGTGCGA250.002870283321.51
AGTGTGA23250.021.51
AGTGACG505.3429176E-821.53
GTGCGAC250.002870283321.52
GTGTGAC23300.021.52
GGGTGAC353.6510995E-521.52
GCGTGAC404.136913E-621.4999982
AGCGTGA404.136913E-621.4999981
CGTGACG404.136913E-621.4999983
TGGTTTT850.020.9189218
GAACGTG950.020.918929
GAACGGT950.020.918929
GAACGGC850.020.918929
GAACGCG950.020.918929
Produced by FastQC (version 0.11.2)