FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_76.3510000005d2a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_76.3510000005d2a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25727
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGACGAGATCTGTCTCTTATACAC1530.5947059509464765No Hit
AGAGGACGAGCTGTCTCTTATACACAT970.37703579896606676No Hit
AGAGGACGAGATCTGTCTCTTCTACAC500.19434834998250863No Hit
AGAGGACGAGATGGGGGCTGTCTCTTA300.11660900998950519No Hit
AGAGGACGAGCTGTCTCTTATCCACAT300.11660900998950519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGGAC22200.021.5450921
AGTGGAC404.059726E-621.545091
AGCGGAC1550.021.545091
AGAGAAC404.059726E-621.545091
AGGACGA22450.021.5023
CGGACGA1550.021.5023
GCGGACG1550.021.5022
AGAACGA404.1313815E-621.5019993
TGGACGA404.1313815E-621.5019993
GAGGACG22300.021.4537892
TGATTTT456.1319224E-720.91634418
GATGTGC456.1319224E-720.91634410
CCGTTTT456.1319224E-720.91634418
GAGTTTT456.1319224E-720.91634418
ACGAGAT25450.020.9163446
AGATGTG1350.020.9163449
AGATGTA456.1319224E-720.9163449
GACGAGA24950.020.9163445
AGATGAG456.1319224E-720.9163449
CGAGATG9850.020.9163447
Produced by FastQC (version 0.11.2)