FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_73.3510000005d236.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_73.3510000005d236.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26121
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTCGTTGCTGTCTCTTATACAC460.1761035182420275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTCG23700.021.315793
TCGGTTC404.4625594E-621.315792
CTTGGTT23100.021.315791
CGTGGTT303.4273593E-421.315791
TTGGTTC23100.021.315792
GTGGTTC303.4273593E-421.315792
CGGTTCG404.4625594E-621.315793
TGATTCG701.0913936E-1121.3157883
TTGATTC701.0913936E-1121.3157882
TGGTTTT405.1943116E-620.94827718
AACGTTT354.4695982E-520.94827717
GTTGATA354.4695982E-520.9482779
GTTGAGT354.4695982E-520.9482779
GTTGAGG800.020.9482779
GTTGAGA405.1943116E-620.9482779
GTTGACC354.4695982E-520.9482779
GTTGAAA405.1943116E-620.9482779
AGCTTTT303.8571912E-420.94827718
ACGTTTT701.2732926E-1120.94827718
GGCGTTT405.1943116E-620.94827717
Produced by FastQC (version 0.11.2)