FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_70.3510000005d1d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_70.3510000005d1d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45132
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGAACTACCCCTGTCTCTTATACA1280.283612514402198No Hit
GTCAGAACTACCGCTGTCTCTTATACA1130.2503766728706904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAAAA557.619747E-921.1814121
CCAGAAC404.846139E-621.1814122
GCCAGAA404.846139E-621.1814121
TCAAAAC557.619747E-921.1814122
TCAGAAC39650.021.1814122
GCAGAAC455.6224417E-721.1814122
CAAAACT557.619747E-921.1814123
TTAGAAC701.2732926E-1121.181412
TAGAACT701.2732926E-1121.181413
GTCCGAA2450.021.181411
GTTAGAA701.2732926E-1121.181411
CAGAACT40700.021.181413
TCCGAAC2450.021.181412
GTCAGAA39550.021.181411
CCGAACT2450.021.181413
ACCGGTC507.2743205E-820.97006810
TGCATTT250.00335801520.97006817
ACCGGGA507.2743205E-820.97006810
ACCGGCA354.5275476E-520.97006810
ACCGGAC303.8907185E-420.97006810
Produced by FastQC (version 0.11.2)