FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_7.3510000005ca0a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_7.3510000005ca0a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10855
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAGCAATGCCTGTCTCTTATACAC540.4974666052510364No Hit
CTTCAGCAATGCCTGTCTCTTCTACAC120.11054813450023032No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGCAA353.840011E-521.1674423
TTCCGCA900.021.1674422
CTCCAGC353.840011E-521.1674421
TCCAGCA353.840011E-521.1674422
TCAGCAA9100.021.1674423
TCCGCAA900.021.1674423
TTCAGCA8850.021.1674422
CTTCCGC900.021.1674421
CTTCAGC8900.021.167441
GTGTTTT250.00319589620.97235118
GGGTTTT850.020.97235118
CAATGCT2100.020.9723517
CAATGCC1800.020.9723517
CAGCAAT9750.020.9723514
TGCGCGG250.00319589620.97235110
TTTCTGT303.6307634E-420.97235121
TTATTTT250.00319589620.97235118
TTCTGTC303.6307634E-420.97235121
GGTTTTT1500.020.97235119
CATTTTT455.341881E-720.97235119
Produced by FastQC (version 0.11.2)