FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_64.3510000005d12a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_64.3510000005d12a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34937
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACACCATAGCCTGTCTCTTATACAC2890.8272032515671065No Hit
GAACACCATAGCGGCTGTCTCTTATAC1930.5524229327074448No Hit
GAACACCATAGCGCCTGTCTCTTATAC1160.3320262186220912No Hit
GAACACCATAGCGTCTGTCTCTTATAC960.27478031885966164No Hit
GAACACCATAGCCTGTCTCTTCTACAC900.2576065489309328No Hit
GAACACCATAGCAGCTGTCTCTTATAC840.24043277900220394No Hit
GAACACCATAGCCCCTGTCTCTTATAC700.20036064916850332No Hit
GAACACCATAGCTGTCTCTTATACACA680.19463605919226035No Hit
GAACACCATAGCTGCTGTCTCTTATAC680.19463605919226035No Hit
GAACACCATAGCATCTGTCTCTTATAC650.18604917422789594No Hit
GAACACCATAGCGACTGTCTCTTATAC580.1660131093110456No Hit
GAACACCATAGCTCCTGTCTCTTATAC580.1660131093110456No Hit
GAACACCATAGCACCTGTCTCTTATAC570.1631508143229241No Hit
GAACACCATAGCCGCTGTCTCTTATAC500.1431147494060738No Hit
GAACACCATAGCCCTGTCTCTTATACA420.12021638950110197No Hit
GAACACCATAGCTTCTGTCTCTTATAC350.10018032458425166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCACCA1250.021.3148692
ACACCAT31000.021.3148693
GTACACC701.0913936E-1121.3148691
AACCCCA1400.021.3148692
TACACCA701.0913936E-1121.3148692
CCACCAT1250.021.3148693
GAACCCC1400.021.3148691
ACCCCAT1400.021.3148693
GAACACC30300.021.3148691
GATCACC353.956702E-521.3148691
ATCACCA353.956702E-521.3148692
TCACCAT353.956702E-521.3148693
GACCACC1250.021.3148691
AACACCA30300.021.3148692
AGGGGTT303.893391E-420.94842516
AGCCCGG303.893391E-420.94842510
TGGTTTT1150.020.94842518
GCGGGGA250.003363032620.94842511
TAGCGGT1150.020.9484259
TAGCGGG2500.020.9484259
Produced by FastQC (version 0.11.2)