FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_61.3510000005d0cb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_61.3510000005d0cb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31647
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTCTATGGTGGCTGTCTCTTATACA1410.44553986159825576No Hit
GACTCTATGGTCTGTCTCTTATACACA1280.40446171833033145No Hit
GACTCTATGGTGCCTGTCTCTTATACA820.2591082883053686No Hit
GACTCTATGGTGTCTGTCTCTTATACA690.2180301450374443No Hit
GACTCTATGGTGACTGTCTCTTATACA360.11375485828040574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCTA455.1689676E-721.3220621
GATTCTA455.1689676E-721.3220621
GACTCTA27450.021.3220621
GACTATA1800.021.3220621
CTATATG1800.021.287783
AATCTAT455.2501855E-721.287782
ATCTATG455.2501855E-721.287783
ACTCTAT27500.021.287782
CTCTATG28150.021.287783
TTCTATG455.2501855E-721.287783
ATTCTAT455.2501855E-721.287782
ACTATAT1800.021.287782
GGTGATC354.5039647E-520.950959
GGTGATA558.403731E-920.950959
GGTGAGT701.2732926E-1120.950959
GGTGAGG701.2732926E-1120.950959
GGTGAAT701.2732926E-1120.950959
ATGGTGC6100.020.950957
CCGTTTT303.8789347E-420.9509518
TAGTTTT609.858923E-1020.9509518
Produced by FastQC (version 0.11.2)