FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_6.3510000005c9ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_6.3510000005c9ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6777
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATCGTAGCCTGTCTCTTATACACA1011.4903349564704147No Hit
CTGATCGTAGCACCTGTCTCTTATACA230.33938320790910437No Hit
CTGATCGTAGCAGCTGTCTCTTATACA170.25084845801977274No Hit
CTGATCGTAGCAGGGGCTGTCTCTTAT100.14755791648221928No Hit
CTGATCGTAGCATCTGTCTCTTATACA90.13280212483399734No Hit
CTGATCGTAGCAGGGGGGCTGTCTCTT80.11804633318577543No Hit
CTGATCGTAGCAGGGGGCGGTTTTTTT70.10329054153755349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGT6000.021.1343292
GATCGTA6150.021.1343293
CTGATCG6000.021.1343291
GCTCGTA303.2567934E-421.1343293
TGCTCGT303.2567934E-421.1343292
CTGCTCG303.2567934E-421.1343291
TGTTTTT656.91216E-1120.9777819
GGTTTTT656.91216E-1120.9777819
CGTAGCC353.8257258E-520.977786
AGCAGGA353.8257258E-520.977789
AGCACGG353.8257258E-520.977789
ATCGTAG6350.020.977784
GTAGCAC1400.020.977787
AGCCTGT250.00306728520.9777779
GTGTTTT303.4285558E-420.97777718
GGGTTTT404.2583288E-620.97777718
TATACAC250.00306728520.97777720
GCAGGGG454.724734E-720.97777710
TAGCCTG250.00306728520.9777778
CATTTTT250.00306728520.97777719
Produced by FastQC (version 0.11.2)