FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_58.3510000005d052.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_58.3510000005d052.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36715
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTCTCTGCATCTGTCTCTTATACAC2080.5665259430750375No Hit
ACGTCTCTGCATCTGTCTCTTCTACAC770.20972354623450903No Hit
ACGTCTCTGCCTGTCTCTTATACACAT760.20699986381587906No Hit
ACGTCTCTGCATGGGGGCTGTCTCTTA390.10622361432656952No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCT250.003120342721.223452
ACGTTTC250.003120342721.223451
GTATCTG2400.021.223453
CGTATCT2400.021.223452
ACGTCTC31950.021.2234481
ACTTCTC354.098371E-521.2234481
TTCTCTG354.098371E-521.2234483
GTCTCTG32450.021.2234483
CGTGTCT455.457023E-721.2234482
ACGTGTC455.457023E-721.2234481
CTTCTCT354.098371E-521.2234482
CGTCTCT32000.021.1902872
ATGGGTG250.00335256520.96321711
GGAGATT250.00335256520.96321716
TTGTTTT507.2061084E-820.96321718
AACGTTT250.00335256520.96321717
ATGGGGA250.00335256520.96321711
CATGATG250.00335256520.96321710
GGGTTTT2250.020.96321718
TGCATGT3200.020.9632178
Produced by FastQC (version 0.11.2)