FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_56.3510000005d01e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_56.3510000005d01e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27635
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTGCTCATGCTGTCTCTTATACAC4931.7839696037633437No Hit
ACAGTGCTCATGCTGTCTCTTCTACAC1620.5862131355165551No Hit
ACAGTGCTCATGGGCTGTCTCTTATAC1290.4667993486520717No Hit
ACAGTGCTCATGGCTGTCTCTTATACA640.23159037452505882No Hit
ACAGTGCTCATGAGCTGTCTCTTATAC500.18092998009770217No Hit
ACAGTGCTCATGGCCTGTCTCTTATAC470.17007418129184004No Hit
ACAGTGCTCATGGTCTGTCTCTTATAC440.15921838248597792No Hit
ACAGTGCTCATGGGGGGCTGTCTCTTA330.11941378686448345No Hit
ACAGTGCTCATGTGCTGTCTCTTATAC300.1085579880586213No Hit
ACAGTGCTCATGCCTGTCTCTTATACA300.1085579880586213No Hit
ACAGTGCTCATGGGGGGGCTGTCTCTT290.10493938845666728No Hit
ACCGTGCTCATGCTGTCTCTTCTACAC280.10132078885471323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTGC24700.021.365991
ACTGTGC250.002981973821.365991
CCGTGCT1450.021.365992
ACCGTGC1450.021.365991
AGTGCTC24900.021.365993
CTGTGCT250.002981973821.365992
CGTGCTC1450.021.365993
CAGTGCT24750.021.365992
TGTGCTC250.002981973821.365993
ACAGCGC303.3799463E-421.3659881
AGCGCTC303.3799463E-421.3659883
CAGCGCT303.3799463E-421.3659882
TTGTTTT751.8189894E-1220.94021818
CATGATG507.125345E-820.9402189
GTGTCTG250.0033529920.94021818
GCCTTTT354.494997E-520.94021818
CATGAGG800.020.9402189
CATGAGC456.0989987E-720.9402189
GGCATTT250.0033529920.94021817
CATGACG507.125345E-820.9402189
Produced by FastQC (version 0.11.2)