FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_55.3510000005cff6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_55.3510000005cff6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37437
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAGCAATGCCTGTCTCTTATACAC1170.31252504207067877No Hit
CTTCAGCAATGCCTGTCTCTTCTACAC490.13088655608088257No Hit
CTTCAGCAATGCGGGGGGGGTTTTTTT460.12287309346368566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCAGC30600.021.2439021
CCAGCAA800.021.2439023
TTCCGCA2800.021.2439022
CTCCAGC800.021.2439021
TCCAGCA800.021.2439022
TCAGCAA31500.021.2439023
TCCGCAA2800.021.2439023
CTGCAGC1200.021.2439021
TTCAGCA30600.021.2439022
GTCAGCA608.421921E-1021.2439022
CTTCCGC2800.021.2439021
TTGTTTT751.8189894E-1220.95989418
CAATGCC7550.020.9598947
TGCGCGT303.8862444E-420.95989410
TGCGTGC303.8862444E-420.95989410
TGCGCAG303.8862444E-420.95989410
CGCTTTT303.8862444E-420.95989418
TGTTTTT2600.020.95989419
TGCTGGG651.1823431E-1020.95989410
GGTTCTG303.8862444E-420.95989419
Produced by FastQC (version 0.11.2)