FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_54.3510000005cfdc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_54.3510000005cfdc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22894
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATCGTAGCCTGTCTCTTATACACA2601.135668734166157No Hit
CTGATCGTAGCACCTGTCTCTTATACA740.32322879357036777No Hit
CTGATCGTAGCAGCTGTCTCTTATACA510.2227657901633616No Hit
CTGATCGTAGCAGGGGGGGGTTTTTTT360.15724644011531405No Hit
CTGATCGTAGCAGGGGCTGTCTCTTAT320.13977461343583472No Hit
CTGATCGTAGCATCTGTCTCTTATACA270.11793483008648553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGTA1150.021.1189443
TGCTCGT1150.021.1189442
CTGCTCG1150.021.1189441
TGATCGT20500.021.1189422
GATCGTA21100.021.1189423
CTGATCG20500.021.1189421
TAGCAGG5650.020.9803098
AGGGGTT354.3861175E-520.98030716
GGGTTTT1900.020.98030718
GCCTTTT250.003298682420.98030718
GCAAGGG354.3861175E-520.98030710
TATACAC250.003298682420.98030720
GGCGTTT250.003298682420.98030717
TTTCTGT455.8943806E-720.98030721
TCGGTTT250.003298682420.98030717
GTTCTGT455.8943806E-720.98030720
GCAGGCG250.003298682420.98030710
GCGGTTT250.003298682420.98030717
TGTTTTT1700.020.98030719
AGGGTTT354.3861175E-520.98030717
Produced by FastQC (version 0.11.2)