FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_50.3510000005cf62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_50.3510000005cf62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48230
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAGTGAGCTAGGGCTGTCTCTTATA490.10159651669085631No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTGA303.5658063E-421.2460582
GCAGTGA1050.021.2460582
GGAAGTG455.475522E-721.2460581
TACTGAG303.5658063E-421.2460583
GGTACTG303.5658063E-421.2460581
CAGTGAG1050.021.2460583
GTAGTGA42000.021.2460582
TAGTGAG42200.021.2460563
TCGTGAG1650.021.2460563
GGTAGTG42050.021.2460561
GTAATGA404.7305766E-621.2460562
GGTAATG404.7305766E-621.2460561
GGTCGTG1650.021.2460561
AGGGGTT303.909327E-420.95954516
CTACAGG303.909327E-420.95954510
GTGTTTT1200.020.95954518
ACGCTTT250.003371131820.95954517
CTGAGCT303.909327E-420.9595455
TCGATTT250.003371131820.95954517
GAGTGTT250.003371131820.95954516
Produced by FastQC (version 0.11.2)