FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_48.3510000005cf12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_48.3510000005cf12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8157
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGCGTGGAACCTGTCTCTTATACAC971.1891626823587105No Hit
TAAGCGTGGAACCTGTCTCTTCTACAC360.4413387274733358No Hit
TAAGCGTGGAACTGTCTCTTATACACA110.13485350006129704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCGT7150.021.2236021
AAGAGTG250.002915760321.2236022
TAAGAGT250.002915760321.2236021
AGAGTGG250.002915760321.2236023
AAGCGTG7150.021.2236022
AGCGTGG7350.021.2236023
TACGCGT303.245237E-421.22361
CGCGTGG303.245237E-421.22363
TGGAACA1250.020.9631927
GTGTTTT303.5331518E-420.9631918
GGGTTTT455.025322E-720.9631918
AACCGGG250.00313577320.9631910
AATTTTT250.00313577320.9631919
GAGTGGA250.00313577320.963194
TTATTTT303.5331518E-420.9631918
TGTTTTT751.8189894E-1220.9631919
GAACGGT404.4758926E-620.963199
GAACGGG657.8216544E-1120.963199
GAACGGC250.00313577320.963199
GAACGCT250.00313577320.963199
Produced by FastQC (version 0.11.2)