FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_41.3510000005ce3d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_41.3510000005ce3d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7961
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAACCGATAGGCTGTCTCTTATACAC700.8792865217937444No Hit
TCAACCGATAGGCTGTCTCTTCTACAC210.2637859565381233No Hit
TCAACCGATAGGGGCTGTCTCTTATAC150.18841854038437383No Hit
TCAACCGATAGGTGCTGTCTCTTATAC110.13817359628187414No Hit
TCAACCGATAGGACCTGTCTCTTATAC90.11305112423062429No Hit
TCAACCGATAGGGCCTGTCTCTTATAC90.11305112423062429No Hit
TCAACCGATAGGGCTGTCTCTTATACA80.10048988820499938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAT7500.021.1139243
CAACCGA7400.021.1139242
TCAACCG7400.021.1139241
CGATAGG7950.020.9811336
TAGGAGT303.5020203E-420.9811339
ATAGGTT556.162736E-920.9811338
ATAGGTG454.951071E-720.9811338
ATAGGGT505.5320925E-820.9811338
ATAGGGC404.4193403E-620.9811338
TTTTTTT3200.020.98113321
TGTTTTT303.5020203E-420.98113319
ATAGGCA606.8394E-1020.9811338
ATAGGAT556.162736E-920.9811338
ATAGGAG800.020.9811338
ATAGGAC606.8394E-1020.9811338
GGTTTTT505.5320925E-820.98113319
ACCGATA7850.020.9811334
GATTTTT404.4193403E-620.98113319
AGGTTAA250.003113434420.98113310
TAGGGTG250.003113434420.9811339
Produced by FastQC (version 0.11.2)