FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_40.3510000005ce20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_40.3510000005ce20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5394
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGCTTGACCTGTCTCTTATACAC340.6303299962921765No Hit
GTTGAGCTTGACCTGTCTCTTCTACAC140.25954764553207266No Hit
GTTGAGCTTGACACCTGTCTCTTATAC80.14831294030404152No Hit
GTTGAGCTTGACGGCTGTCTCTTATAC70.12977382276603633No Hit
GTTGAGCTTGACGGGCGGGGTTTTTTT60.11123470522803114No Hit
GTTGAGCTTGACGCTGTCTCTTATACA60.11123470522803114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGAGC4250.021.3428571
TTGAGCT4250.021.3428572
TGAGCTT4400.021.3428573
TGCGCTT503.880632E-821.3428553
TTGCGCT503.880632E-821.3428552
GTTGCGC503.880632E-821.3428551
AGCTTGA4800.020.9439265
TGACTGG303.3339197E-420.9439269
GAGCTTG4800.020.9439264
CATTTTT303.3339197E-420.94392619
GAGTTTT303.3339197E-420.94392618
TTGACTA303.3339197E-420.9439268
TTGACGG1250.020.9439268
TTGACGA303.3339197E-420.9439268
GATTTTT454.409012E-720.94392619
TTGACCC303.3339197E-420.9439268
GCTTGAC5350.020.9439266
GGGGTTT303.3339197E-420.94392617
CTTGACT1350.020.9439267
CTTGACC850.020.9439267
Produced by FastQC (version 0.11.2)