FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_34.3510000005cd58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_34.3510000005cd58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8157
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCGTCTCGATCTGTCTCTTATACAC650.7968615912713007No Hit
ACTCGTCTCGATCTGTCTCTTCTACAC180.2206693637366679No Hit
ACTCGTCTCGATGCTGTCTCTTATACA120.1471129091577786No Hit
ACTCGTCTCGATCCTGTCTCTTATACA90.11033468186833395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGTC7150.021.2236021
TCGTCTC7250.021.2236023
CTCGTCT7200.021.2236022
ACCCGTC303.245237E-421.22361
CCGTCTC303.245237E-421.22363
CCCGTCT303.245237E-421.22362
ATTTTTT1750.020.96319220
CTCGATT1750.020.9631927
GATGGGC250.00313577320.9631910
GGGTTTT250.00313577320.9631918
TCGATTG850.020.963198
TCGATTA404.4758926E-620.963198
TCGATGT751.8189894E-1220.963198
TCGATGG1700.020.963198
TCGATGC751.8189894E-1220.963198
TCGATGA556.2846084E-920.963198
TCGATCT250.00313577320.963198
TCGATCG455.025322E-720.963198
TCGATCC303.5331518E-420.963198
TTTCTGT250.00313577320.9631921
Produced by FastQC (version 0.11.2)