FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_33.3510000005cd3e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_33.3510000005cd3e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6776
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGGAATGGCCTGTCTCTTATACACA340.5017709563164109No Hit
AACGGAATGGCTGCTGTCTCTTATACA240.3541912632821724No Hit
AACGGAATGGCTCTGTCTCTTATACAC170.25088547815820544No Hit
AACGGAATGGCTGTCTCTTATACACAT160.23612750885478156No Hit
AACGGAATGGCTCCTGTCTCTTATACA130.19185360094451004No Hit
AACGGAATGGCTACTGTCTCTTATACA80.11806375442739078No Hit
AACGGAATGGCTTCTGTCTCTTATACA80.11806375442739078No Hit
AACGGAATGGCTGGGGGGGGTTTTTTT70.10330578512396695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATG6300.021.2706783
AACGGAA6250.021.2706761
ACGGAAT6250.021.2706762
AATTTTT404.2976826E-620.95555719
TTTCTGT353.8571627E-520.95555721
ATGGCTC1600.020.9555577
CATTTTT404.2976826E-620.95555719
GGCTGGG404.2976826E-620.9555579
GAATGGC6400.020.9555575
AATGGCT6650.020.9555576
GGCTAAG404.2976826E-620.9555579
GGAATGG6450.020.9555574
AGTTTTT353.8571627E-520.95555719
TGGCTTA353.8571627E-520.9555578
TGGCTGA404.2976826E-620.9555578
TGGCTCG404.2976826E-620.9555578
TGGCTAC353.8571627E-520.9555578
TGATTTT303.4532213E-420.95555518
ATGGCTT900.020.9555557
ATGGCTG2600.020.9555557
Produced by FastQC (version 0.11.2)