FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_32.3510000005cd14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_32.3510000005cd14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9606
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAGTTAGCCCTGTCTCTTATACAC510.5309181761399125No Hit
GGATAGTTAGCCTGTCTCTTATACACA190.1977930460129086No Hit
GGATAGTTAGCCCTGTCTCTTCTACAC130.13533208411409536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTT656.366463E-1121.3829782
GGATCGT656.366463E-1121.3829781
GGATAGT7850.021.3829781
ATAGTTA7850.021.3829783
GCTAGTT403.8750586E-621.3829772
GGTTAGT403.8750586E-621.3829771
CTAGTTA403.8750586E-621.3829773
GGCTAGT403.8750586E-621.3829771
TTAGTTA403.8750586E-621.3829773
GATAGTT7850.021.2467822
CCTTTTT556.7593646E-920.93750219
GATTTTT556.7593646E-920.93750219
AGCCCGG250.003199749620.93759
AGCCTGG404.6692912E-620.93759
AGCCTGA303.6285704E-420.93759
TGGTTTT303.6285704E-420.937518
TCCTTTT250.003199749620.937518
AGCCTAG250.003199749620.93759
GTGTTTT250.003199749620.937518
GGGTTTT505.986476E-820.937518
Produced by FastQC (version 0.11.2)