FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_31.3510000005ccf9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_31.3510000005ccf9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11052
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACATCGCAAAAAAAAAAAAAAAAA170.15381831342743396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATCG10500.021.0818183
AGACATC10350.021.0818182
GTGTTTT455.3263466E-720.98642518
GCAAGTG455.3263466E-720.9864259
GCAAGGT303.6202854E-420.9864259
GCAAGGG607.7852746E-1020.9864259
GCAAGCG303.6202854E-420.9864259
GCAAGCA354.1167856E-520.9864259
AATTTTT800.020.98642519
TGATTTT303.6202854E-420.98642518
TGTTTTT850.020.98642519
TTCTGTC404.6832956E-620.98642521
GCAATTT303.6202854E-420.9864259
GCAATTG303.6202854E-420.9864259
GCAATGG404.6832956E-620.9864259
ACTTTTT900.020.98642519
ACATCGC10650.020.9864254
GCAATAG303.6202854E-420.9864259
GCAAATT303.6202854E-420.9864259
TAATTTT303.6202854E-420.98642518
Produced by FastQC (version 0.11.2)