FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_30.3510000005ccdf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_30.3510000005ccdf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6777
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGTGACGAACCTGTCTCTTATACAC480.7082779991146525No Hit
AGTGTGACGAACCTGTCTCTTCTACAC120.17706949977866313No Hit
AGTGTGACGAACGGGGGGGGTTTTTTT70.10329054153755349No Hit
AGTGTGACGAACTGTCTCTTATACACA70.10329054153755349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGACG6150.021.3787883
AGTGTGA6050.021.3787881
GTGTGAC6050.021.3787882
GTATTTT250.002996298721.05970218
GTTTCTG250.002996298721.05970220
GTTTTTT2150.021.05970220
GGTTTTT850.021.05970219
CATTTTT250.002996298721.05970219
TATTTTT454.5395973E-721.05970219
GCGTTTT250.002996298721.05970218
ATTTTTT1350.021.05970220
AGTTTTT555.475158E-921.05970219
AAGTTTT250.002996298721.05970218
CTTTTTT1100.021.05970220
CGTTTTT404.1088024E-621.05970219
GGGTTTT303.335664E-421.059718
AATTTTT303.335664E-421.059719
TTTCTGT353.706811E-521.059721
TTTTTTT4550.021.059721
TGTTTTT353.706811E-521.059719
Produced by FastQC (version 0.11.2)