FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_3.3510000005c982.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_3.3510000005c982.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8026
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAACAGAGTCTGTCTCTTATACAC1321.644654871667082No Hit
GAGGAACAGAGCTGTCTCTTATACACA510.6354348367804635No Hit
GAGGAACAGAGTCTGTCTCTTCTACAC450.5606777971592325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGAAC6900.021.227851
AGGAACA6900.021.227852
GAGGCAC454.4159788E-721.2278481
GGCACAG454.4159788E-721.2278483
AGGCACA454.4159788E-721.2278482
GGAACAG7050.021.2278463
ACAGAGT7850.020.9625026
CAGAGTA1450.020.9625027
GAGTGTT250.00313127220.96259
GAGTGGG657.6397555E-1120.96259
GAGTGGC303.5258717E-420.96259
GAGTGCC250.00313127220.96259
GAGTGAG250.00313127220.96259
AGTAGGG250.00313127220.962510
TTTTTTT3250.020.962521
TGTTTTT250.00313127220.962519
AGTGGGG353.967908E-520.962510
AGTGGGC250.00313127220.962510
GTTTTTT1500.020.962520
GGTTTTT657.6397555E-1120.962519
Produced by FastQC (version 0.11.2)