FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_29.3510000005ccc2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_29.3510000005ccc2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18291
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTCCAAGCCTGTCTCTTATACACA1981.0824995899622765No Hit
AAGGTCCAAGCACTGTCTCTTATACAC1390.7599365808321032No Hit
AAGGTCCAAGCACTGTCTCTTCTACAC610.33349734842272155No Hit
AAGGTCCAAGCAGTGGTATCAACGCAG390.21321961620469082No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCCA250.002954168521.3519552
GGTCCAA17200.021.3519553
ATGGTCC250.002954168521.3519551
AAGGTCC17000.021.3519551
AGGTCCA17000.021.3519552
TTGTTTT354.3842047E-520.94246718
TGGTTTT250.003307875220.94246718
GGGTTTT506.787013E-820.94246718
AAATTTT250.003307875220.94246718
TTTCTGT250.003307875220.94246721
GCAGGTG250.003307875220.94246710
TTATTTT250.003307875220.94246718
CGCTTTT250.003307875220.94246718
GGTTTTT1650.020.94246719
CATTTTT1000.020.94246719
AGCAGCT506.787013E-820.9424679
AGCAGAC354.3842047E-520.9424679
GCAGTGC250.003307875220.94246710
GTCTTTT250.003307875220.94246718
GCGCTGT250.003307875220.94246718
Produced by FastQC (version 0.11.2)