FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_27.3510000005cc8f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_27.3510000005cc8f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8092
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGACCAACGACTGTCTCTTATACAC130.1606524962926347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCA7050.021.3417722
TTCGCCC454.1938983E-721.3417721
CGCCCAA454.1938983E-721.3417723
TTCGACC7050.021.3417721
TCGCCCA454.1938983E-721.3417722
CGACCAA7150.021.1925283
CCAACGA7850.020.9441016
AACGAGG1050.020.9441018
AACGAGA751.8189894E-1220.9441018
GCTTTTT751.8189894E-1220.94410119
TTGTTTT250.003149000720.944118
CAGTTTT404.4996414E-620.944118
GCCCAAC455.053025E-720.94414
AACGATT505.6599674E-820.94418
AACGATC404.4996414E-620.94418
AATTTTT556.320988E-920.944119
AACGAGC607.039489E-1020.94418
AACGACT303.5496047E-420.94418
AACGACG505.6599674E-820.94418
AACGACA250.003149000720.94418
Produced by FastQC (version 0.11.2)