FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_26.3510000005cc65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_26.3510000005cc65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12697
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTGTGTCCTGTCTCTTATACAC1541.212884933448846No Hit
ACTGTCTCTGTCTCTTATACACATCTC1481.1656296763014886No Hit
ACTGTCTGTGTCCTGTCTCTTCTACAC460.3622903047964086No Hit
ACTGTCTCTGTCTCTTATACCCATCTC200.15751752382452547No Hit
ACTGTCTGTGTCTGTCTCTTATACACA150.1181381428683941No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCCT250.002954067221.2891561
TGCCTGT250.002954067221.2891563
CTGCCTG250.002954067221.2891562
CTGTCTG11300.021.1949562
ACTGTCT11800.021.1087421
GTGTCTT250.003243157620.952578
GTGTCTG659.458745E-1120.952578
GTGTCTA659.458745E-1120.952578
GGGTTTT557.2523108E-920.9525718
GTGTCGT701.0913936E-1120.952578
GTGTCGG1850.020.952578
GTGTCGC1000.020.952578
GTGTCGA1100.020.952578
GTGTCCT659.458745E-1120.952578
GTGTCCG800.020.952578
GTGTCCC506.342816E-820.952578
GTGTCCA659.458745E-1120.952578
GTGTCAT850.020.952578
GTGTCAG1100.020.952578
GTGTCAC557.2523108E-920.952578
Produced by FastQC (version 0.11.2)