FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_25.3510000005cc4c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_25.3510000005cc4c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTCGTTGCTGTCTCTTATACAC80.64No Hit
CTTGGTTCGTTGGGGGTCTGTCTCTTA20.16No Hit
CTTGGTTCGTTGGGGGGGGGTCTGTCT20.16No Hit
CTTGGTTCGTTGTCTGTCTCTTATACA20.16No Hit
CTTGGTTCGTTGGGGCGGGGTTTTTTT20.16No Hit
CTTGGTTCGTTGAACAGCGATTTTTTT20.16No Hit
CTTGGTTCGTTGGGGGGGCCTTTTTTT20.16No Hit
CTTGGTTCGTTGACCGCCTATTTTTTT20.16No Hit
CTTGGTTCGTTGGAGGTGCATTTTTTT20.16No Hit
CTTGGTTCGTTGGAACTATCTTTTTTT20.16No Hit
CTTGGTTCGTTGATGGGTGGTTTTTTT20.16No Hit
CTTGGTTCGTTGTCTTTACGTTTTTTT20.16No Hit
CTTGGTTCGTTGGTGCATCATTTTTTT20.16No Hit
CTTGGTTCGTTGGGGCGGGATTTTTTT20.16No Hit
CTTGGTTCGTTGTGCGCTGCTTTTTTT20.16No Hit
CTTGGTTCGTTGACAGGGTGTTTTTTT20.16No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGT459.277028E-821.0000027
CGTTGTG301.6740938E-421.0000028
TGGTTCG1150.021.03
TTCGTTG1250.021.06
GGTTCGT1150.021.04
GTTCGTT1150.021.05
CTTTTTT351.44566875E-521.020
TTTTTTT1000.020.99999821
CTTGGTT1000.020.9999981
TCGTTGG506.835762E-920.9999987
TTGGTTC1000.020.9999982
CGTTGGG250.001853412620.9999988
ATTTTTT250.001853412620.99999820
GTTTTTT453.338244E-618.66666820
Produced by FastQC (version 0.11.2)