FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_24.3510000005cc22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_24.3510000005cc22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24082
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGTGTGGAGGCTGTCTCTTATAC1780.7391412673365999No Hit
GTGTAGTGTGGCTGTCTCTTATACACA1610.6685491238269247No Hit
GTGTAGTGTGGAGTCTGTCTCTTATAC680.2823685740387011No Hit
GTGTAGTGTGGAGCCTGTCTCTTATAC600.24914874179885393No Hit
GTGTAGTGTGGATGCTGTCTCTTATAC570.2366913047089112No Hit
GTGTAGTGTGGAAGCTGTCTCTTATAC530.22008138858898765No Hit
GTGTAGTGTGGAGCTGTCTCTTATACA480.19931899343908316No Hit
GTGTAGTGTGGAGACTGTCTCTTATAC460.19101403537912134No Hit
GTGTAGTGTGGACCTGTCTCTTATACA410.17025164022921685No Hit
GTGTAGTGTGGACGCTGTCTCTTATAC380.15779420313927414No Hit
GTGTAGTGTGGAACCTGTCTCTTATAC260.10796445477950335No Hit
GTGTAGTGTGGAGGGGCTGTCTCTTAT260.10796445477950335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGT21300.021.2658231
TGTAGTG21300.021.2658232
GTGTCGT1350.021.2658211
GTCGTGT1350.021.2658213
TGTCGTG1350.021.2658212
GTAGTGT21900.021.217273
TTGTTTT651.10958354E-1020.9563418
TGGTTTT651.10958354E-1020.9563418
GTGTTTT800.020.9563418
AATTTTT950.020.9563419
TTTCTGT800.020.9563421
GAAGTTT405.1488696E-620.9563417
GCGGTTT405.1488696E-620.9563417
CATTTTT850.020.9563419
TCGTGTG1350.020.956344
TAGTTTT651.10958354E-1020.9563418
TGGAGTC405.1488696E-620.956349
TGGAGGT900.020.956349
TGGAGGG1650.020.956349
TGGAGGA850.020.956349
Produced by FastQC (version 0.11.2)