FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_23.3510000005cc08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_23.3510000005cc08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14012
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGCTATCCCTGTCTCTTATACACA740.5281187553525549No Hit
ATCTGCTATCCTGTCTCTTATACACAT250.17841849842991722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTAT13100.021.3941613
TCTGCTA13000.021.394162
ATCTGCT13000.021.394161
TGTTTTT1350.020.93571719
TGGTTTT404.939302E-620.93571518
GTGTTTT557.496055E-920.93571518
GGGTTTT608.60382E-1020.93571518
CCGTGGG303.7495847E-420.93571510
CGGGGGT303.7495847E-420.93571511
TTATTTT250.003275775120.93571518
TGATTTT354.3006552E-520.93571518
GTATTTT506.524533E-820.93571518
GGATTTT354.3006552E-520.93571518
GTTTTTT4650.020.93571520
GGTTTTT1850.020.93571519
CATTTTT557.496055E-920.93571519
TAGTTTT303.7495847E-420.93571518
ACTTTTT404.939302E-620.93571519
GCTATCC13650.020.9357155
TCCGGTT404.939302E-620.9357159
Produced by FastQC (version 0.11.2)