FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_21.3510000005cbb6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_21.3510000005cbb6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15131
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTGAACTGGGGCTGTCTCTTATAC280.18505055845614962No Hit
ACCTTGAACTGGAGCTGTCTCTTATAC170.11235212477694798No Hit
ACCTTGAACTGGGCCTGTCTCTTATAC160.10574317626065692No Hit
ACCTTGAACTGGGTCTGTCTCTTATAC160.10574317626065692No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGAA250.002975281621.2962972
CCTTGAA13800.021.2962972
ACATTGA250.002975281621.2962971
ATTGAAC250.002975281621.2962973
ACCTTGA13800.021.2962971
CTTGAAC14000.021.2962973
TTTGAAC454.8578295E-721.2962953
ACTTTGA454.8578295E-721.2962951
CTTTGAA454.8578295E-721.2962952
TTTCTGT354.20706E-521.0132921
TTCTGTC354.20706E-521.0132921
CTTATAC404.8237926E-621.0132921
GTTTCTG250.003217443621.01328820
ATTTTTT1750.021.01328820
ACTGGGT1050.020.943718
ACTGGGG2450.020.943718
TTGTTTT455.718175E-720.94370818
GTGTTTT250.003280447320.94370818
AATCTGT455.718175E-720.94370819
AATTTTT354.3173022E-520.94370819
Produced by FastQC (version 0.11.2)