FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_20.3510000005cb9d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_20.3510000005cb9d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19937
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTCCTACCTGTCTCTTATACACAT1860.9329387570848172No Hit
AGTCTCCTACGTCTGTCTCTTATACAC1530.7674173646988012No Hit
AGTCTCCTACCTGTCTCTTATCCACAT550.27586898731002657No Hit
AGTCTCCTACGTCTGTCTCTTCTACAC410.20564779054020166No Hit
AGTCTCCTACGCTGTCTCTTATACACA400.20063199077092844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACCT404.361098E-621.322252
ACTCCTA505.6646968E-821.322253
GTCTCCT17450.021.322252
GGCTCCT353.8349062E-521.322252
AGGCTCC353.8349062E-521.322251
TCACCTA404.361098E-621.322253
TCTCCTA17650.021.322253
GCTCCTA353.8349062E-521.322253
AGACTCC505.6646968E-821.322251
AGTCCCC303.3799792E-421.322251
AGTCTCC17450.021.322251
GACTCCT505.6646968E-821.322252
AGTCACC404.361098E-621.322251
TCCCCTA303.3799792E-421.322253
TTGTTTT455.903803E-720.94723718
GGGTTTT751.8189894E-1220.94723718
CCTACGT19450.020.9472376
AATTTTT701.2732926E-1120.94723719
ACGTTTG303.8138672E-420.9472379
ACGTTTA354.402485E-520.9472379
Produced by FastQC (version 0.11.2)