FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_19.3510000005cb73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_19.3510000005cb73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13884
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGAGCGCATTCTGTCTCTTATACAC220.15845577643330452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAGCG12200.021.1309092
TCAGCGC751.8189894E-1221.1309091
AGAGCGC12200.021.1309093
TCAGAGC12300.021.1309091
CCGAGCG505.894435E-821.1309092
AGCGCGC751.8189894E-1221.1309093
CAGCGCG751.8189894E-1221.1309092
TCCGAGC505.894435E-821.1309091
TGGTTTT303.6956044E-420.9783418
GGGTTTT250.003235602520.9783418
CATTGTG455.5574674E-720.978349
CATTGTC354.2294345E-520.978349
GAGCGCA12900.020.978344
CATTGGT354.2294345E-520.978349
CATTGGC303.6956044E-420.978349
CATTGGA303.6956044E-420.978349
CATTGCG608.367351E-1020.978349
CATTGCA303.6956044E-420.978349
CATTGAT354.2294345E-520.978349
CATTGAG608.367351E-1020.978349
Produced by FastQC (version 0.11.2)